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LIPID COMPOSITION AND VIRULENCE OF MYCOBACTERIUM TUBERCULOSIS H 37 Rv
Author(s) -
Khuller GK,
Taneja R,
Kaur S,
Verma JN
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.58
Subject(s) - cardiolipin , phosphatidylethanolamine , palmitoleic acid , oleic acid , phospholipid , composition (language) , mycobacterium tuberculosis , virulence , elaidic acid , chemistry , biochemistry , fatty acid , food science , biology , microbiology and biotechnology , tuberculosis , linoleic acid , phosphatidylcholine , membrane , gene , medicine , linguistics , philosophy , pathology
Summary The total lipids increased with decrease in total phospholipids in Mycobacterium tuberculosis H 37 RV grown at 30° as compared to 37°. Oleic and elaidic acid supplemented to the growth medium resulted in a higher total lipid and phospholipid content, but the content was lower in cells grown in the presence of palmitoleic acid. Cellular content of mannophosphoinositides decreased, whereas that of phosphatidylethanolamine and cardiolipin increased at the lower temperature. A decrease in mannophosphoinositides and an increase in cardiolipin were noted with all fatty acid supplements; however, phosphatidylethanolamine behaved differently. The unsaturated to saturated fatty acids ratio increased at low temperature with oleic and elaidic acid supplements, but it decreased with palmitoleic acid. Cells from media supplemented with palmitoleic acid were found to be most virulent to guinea‐pigs. Possible correlation of altered lipid composition and virulence of M. tuberculosis H 37 RV has been discussed.