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DETRIMENTAL EFFECT OF HIGH MEDIUM GLUCOSE CONCENTRATION ON SUBSEQUENT ENDOCRINE FUNCTION OF TRANSPLANTED ORGAN‐CULTURED FOETAL MOUSE PANCREAS
Author(s) -
Collier SA,
Mandel TE,
Carter WM
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.49
Subject(s) - islet , medicine , endocrinology , in vitro , insulin , transplantation , renal capsule , tissue culture , pancreas , biology , organ culture , endocrine system , kidney , hormone , biochemistry
Summary Single 17‐day foetal mouse pancreases, cultured for 15 days in RPMI 1640 or DME containing either 1 or 4 g/1 glucose, were grafted under the kidney capsule of syngeneic mice made diabetic with Streptozotocin. Islets cultured in media containing 1 g/l glucose secreted less insulin in vitro than those grown in media containing 4 g/1 glucose. After transplantation, the blood glucose levels of diabetic mice grafted with islets grown in media containing 1 g/l glucose decreased to normal limits, whereas mice grafted with islets grown in media with 4 g/l glucose never became normoglycaemic. Histologically, grafts of tissue from high glucose cultures were smaller and had less well organized islet tissue than grafts from tissue maintained in physiological glucose concentrations. Islets cultured in DME secreted more insulin in vitro than those cultured in RPMI 1640, but the type of culture medium had no significant effect on subsequent graft function. These data support the concept that chronic exposure of foetal islets to high glucose concentrations may be deleterious to their subsequent function as grafts despite the fact that they secrete more insulin in vitro .