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THE ISOLATION AND TRANSPLANTATION OF FOETAL MOUSE PROISLETS
Author(s) -
Simeonovic Charmaine J,
Lafffrty Kevin J
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.42
Subject(s) - islet , transplantation , pancreas , streptozotocin , isolation (microbiology) , fetus , granulation tissue , biology , pathology , anatomy , andrology , diabetes mellitus , medicine , immunology , endocrinology , pregnancy , wound healing , bioinformatics , genetics
Summary A method is described for the isolation of ‘proislets’ from mouse foetal pancreas. Histologically, isolated proislets are composed of undifferentiated tissue which does not slain with aldehyde fuchsin. Following isotransplantation. proislets develop into well differentiated islets with β cell granulation. In addition, isografts consisting of proislets harvested from 8 foetal mouse pancreases have the functional capacity to reverse Streptozotocin‐induced diabetes. Proislets appear to represent foetal precursor islet tissue.