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SKIN TESTS FOR COELIAC DISEASE EMPLOYING FRACTIONS OF A GLIADIN DIGEST
Author(s) -
Cornell HJ,
Burns E,
Hill DJ
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.38
Subject(s) - gliadin , coeliac disease , gluten , sephadex , prednisolone , dermatitis herpetiformis , gastroenterology , etiology , erythema , medicine , gluten free , disease , chemistry , immunology , pathology , biochemistry , enzyme
Summary Gliadin sub‐fractions have been tested by intradermal injection in 19 adult patients with coeliac disease in remission and in 19 healthy adult controls. Fraction 3, obtained by SP Sephadex chromatography of a peptic‐tryptic digest of gliadin, produced strong reactions (areas of erythema greater than 1 cm 2 ) in 13 of the patients. Weak or negative reactions to fraction 3 were produced in the remaining patients, including 2 patients who were currently on prednisolone therapy in addition to a gluten‐free diet. There was no correlation between time on a gluten‐free diet and severity of reaction in the patients not on prednisolone therapy. A strong reaction was observed with only one control and in the remaining controls all reactions were negative. Other fractions produced strong reactions in comparatively few patients. Skin tests using Fraction 3 could thus form part of the diagnosis for coeliac disease and could lead to a knowledge of the composition of the peptides which are important in the aetiology of this disease.