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TWO ANTI‐TUMOUR CYTOTOXIC CELLS IN THE PERITONEAL CAVITY OF RATS: NATURAL OCCURRENCE, AUGMENTATION AND PARTIAL CHARACTERIZATION
Author(s) -
Inoue Y,
Nelson DS
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.3
Subject(s) - cytotoxic t cell , cytotoxicity , fibrosarcoma , peripheral blood mononuclear cell , peritoneal cavity , microbiology and biotechnology , chemistry , lysis , immune system , biology , immunology , in vitro , biochemistry , anatomy , genetics
Summary Mononuclear cells cytotoxic to methylcholanthrene‐induced fibrosarcoma cells were detected in the peritoneal cavities of normal rats by means of 16 h 51 Cr release and 48 h 125 IUdR release assays. Carriage of a tumour which induces concomitant immunity, or intraperitoneal injections of Corynebacterium parvum or proteose‐peptone, led to increases in mononuclear cell numbers. Injections of C. parvum and proteose‐peptone led to increases in cytotoxicity in 51 Cr release assays. Carriage of an immunogenic tumour led to an increase in cytotoxicity to homologous tumour cells in the 125 IUdR release assay. Cells were separated by sedimentation velocity, metrizamide density gradients and adherence to glass or plastic. Small cells were more active than large cells in 51 Cr release assays. Larger cells tended to be more active in 125 IUdR release assays. Generally, low density cells were more active in both assays, except that high density cells induced by C. parvum were most active in 125 IUdR release. Adherent cells from C. parvum ‐injected rats were more active than non‐adherent cells in both assays. On the basis of differential stimulation and separation by sedimentation velocity it is suggested that two cell types, possibly NK cells and macrophages, are operative. 125 IUdR‐labelled fibrosarcoma cells were lysed after intraperitoneal injection into normal and concomitantly immune rats, more rapidly and extensively in the latter case. These cytotoxic cells may be important in both natural and acquired resistance of rats to tumour growth.