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CHARACTERIZATION OF THE EFFECTOR CELLS RESPONSIBLE FOR TUMOUR RESISTANCE IN SALMONELLA ENTERITIDIS 11RX‐IMMUNIZED MICE
Author(s) -
La Posta Vincent J,
Ashley Michael P,
Kotlarski Ieva
Publication year - 1982
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1982.2
Subject(s) - trypan blue , cytotoxic t cell , in vivo , cytolysis , in vitro , biology , effector , microbiology and biotechnology , cytotoxicity , salmonella enteritidis , salmonella , immunology , biochemistry , bacteria , genetics
Summary In an attempt to characterize the effector cells responsible for tumour resistance in Salmonella enteritidis 11RX‐immunized mice the anti‐macrophage agent trypan blue was used in both in vivo and in vitro experiments. Resistance was measured in vivo by the clearance of 125 I from she peritoneal cavity of mice injected intraperitoneally with 125 I‐5‐iododeoxyuridine labelled Ehrlich Ascites Tumour (EAT) cells. The in vitro correlate was measured by lysis of 51 Cr‐labelled tumour cells by peritoneal cells (PC) from 11RX‐immunized mice. Pre‐treatment of resistant mice with trypan blue greatly reduced both 125 I clearance and 51 Cr release. The in vitro cytolytic activity was non‐specific. Fractionation of cytotoxic PC on the basis of adherence to plastic or nylon wool and buoyant density, coupled with the use of appropriate cell targets, showed that the bulk of cytotoxic activity resided with macrophages, with some contribution from other cells such as natural killer cells. Killing of labelled tumour cells could be inhibited by competition with unlabelled cells or by separating the PC and tumour cells by a cell impermeable membrane. This showed that close association between the effector and target cells was necessary before killing could occur.