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MURINE HYBRIDOMA‐DERIVED ANTIBODIES PRODUCING CIRCUMOVAL PRECIPITATION (COP) REACTIONS WITH EGGS OF SCHISTOSOMA JAPONICUM
Author(s) -
Cruise Kathy M,
Mitchell Graham F,
Tapalés Fe P,
Garcia Edito G,
SungRu Huang
Publication year - 1981
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1981.43
Subject(s) - schistosoma japonicum , antibody , antigen , schistosomiasis , precipitin , radioimmunoassay , biology , immune system , titer , chemistry , microbiology and biotechnology , immunology , helminths , biochemistry
Summary Of 7 hybridomas which secrete immunoglobulins binding to crude extracts of Schistosoma japonicum adult worms and/or eggs in solid‐phase radioimmunoassays (RIAs), 3 gave positive precipitation reactions in the circumoval precipitin test (COPT). The COPT is a simple and inexpensive immunodiagnostic test for schistosomiasis japonica which involves the incubation of a selected batch of S. japonicum eggs with sera from patients and examination for precipitates one or more days later. Using a competitive RIA with an egg antigen extract and a labelled COPT‐positive hybridoma ascites fluid, PwF.41‐1‐3, the surprising observation was made that only one anti‐egg antibody specificity appeared to be represented in the series of 3 antibodies (as ascites fluids). Using sera as inhibitors in the competitive RIA, inhibitory activity (presumably antibodies to the target antigenic determinant of PwF.41‐1‐3) was readily detected in sera from egg‐immunized mice and was of relatively high titre in a strain of mouse (C57BL/6) which can be readily sensitized for large granuloma formation around entrapped eggs in the lungs. Negligible inhibitory activity was found in the sera from S. japonicum ‐infected patients, even with sera from patients with prominent hepatosplenomegaly. The availability of COPT‐positive hybridoma antibodies should facilitate isolation of at least one S. japonicum egg antigen involved in COP reactions and perhaps induction of immunopathological immune responses at least in mice.