Premium
THE ERYTHROCYTE INSULIN RECEPTOR
Author(s) -
McElduff A,
Eastman CJ
Publication year - 1981
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1981.37
Subject(s) - insulin receptor , insulin , receptor , endocrinology , medicine , chemistry , biology , insulin resistance
Summary A precise, reproducible radioreceptor assay has been developed for insulin receptors on human erythrocytes. The mean specific binding in 11 normal volunteers was 7.7% ± 1.6 (S.D.) per 2.25 × 10 9 erythrocytes. The intra and inter assay coefficients of variation are 4%. The erythrocyte insulin receptor is specific for insulin and recognises insulin analogues in proportion to their respective biological activities. The pH and temperature dependence, the negative cooperativity and the inverse relationship between specific binding and fasting plasma Insulin levels suggest it is similar to insulin receptors elsewhere. The erythrocyte insulin receptor offers a model for the study of insulin/insulin receptor interaction in man.