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SUPPRESSOR CELL ACTIVITY IN A PROLIFERATIVE DISORDER OF T LYMPHOCYTES
Author(s) -
Kupa Ann,
Thomas Miriam E,
Moore Helen,
Bradley J,
Zola H,
Hooper M,
Harding P
Publication year - 1981
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1981.19
Subject(s) - phytohaemagglutinin , pokeweed mitogen , concanavalin a , immunology , suppressor , in vivo , in vitro , t cell , medicine , biology , endocrinology , lymphocyte , cancer , immune system , biochemistry , microbiology and biotechnology
Summary We report details of the immunological profile of a patient with the candidiasis endocrinopathy syndrome who has developed T‐type chronic lymphocytic leukaemia. The patient is anergic to a panel of delayed hypersensitivity skin tests, and has poor in vitro mitogenic responses, but B cell function in vivo is not impaired. Subsequent functional studies have revealed that cells from the patient have a significant suppressive effect in coculture (p < 0.05) on the responses of healthy donor lymphocytes (NR) to the mitogen phytohaemagglutinin (PHA). A degree of selectivity for the suppressive effect is suggested by the lack of similar effects on coculture responses to the mitogens cocanavalin A (Con A) and pokeweed mitogen (PWM). Mitomycin C treatment of the patient's cells reduced their suppressive activity but significant suppression was still observed in the majority of PHA cocultures. The suppressor activity required the presence of the pattern's cells in cocultures, as no suppression was observed when the pattern's serum or cell culture supernatant were included instead of the patient's cells in NR cultures.