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URIDINE AND INOSINE: PRESSOR NUCLEOSIDES FROM MAN, RAT AND DOG
Author(s) -
MacDonald GJ,
Burcher E,
Fisher WK,
Bagnara AS,
Barrow KD,
Thompson EOP,
Duffield AM
Publication year - 1981
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1981.12
Subject(s) - inosine , chemistry , uridine , kidney , chromatography , tryptamine , blood pressure , biochemistry , enzyme , endocrinology , biology , rna , gene
Summary Short‐acting pressor compounds isolated from rat kidney, brain, heart and spleen have been identified as inosine and uridine by gas chromatography, mass spectrometry, high pressure liquid chromatography and analysis of ultraviolet spectra. Inosine was further identified by nuclear magnetic resonance. These compounds have also been found in kidneys from hypertensive man, rejected renal transplants and dog and beef kidney. Tissues were extracted by acid ethanol extraction followed by gel filtration and high voltage paper electrophoresis. Compounds found to be pressor in the anaesthetised rat resisted proteolytic enzymes, boiling for 10 min, extremes of pH and incubation with plasma from the source species for up to 20 min. The pressor effects of bolus injections of active gel filtration fractions, uridine and inosine were short‐lived with a maximum effect at 5‐6 s. Intravenous (I.V.) infusions of extracts gave a sustained pressor response without a concurrent change in heart rate. The effect on blood pressure was not accompanied by increased heart rate and persisted when the pressor effects of angiotensin II, noradrenaline and 5‐hydroxy‐tryptamine were blocked pharmacologically.