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AN IMPROVED ASSAY FOR INTERLEUKIN 2 (LYMPHOCYTE GROWTH FACTOR) PRODUCED BY MITOGEN‐ACTIVATED LYMPHOCYTES
Author(s) -
Lafferty KJ,
Prowse SJ,
AlAdra A,
Warren HS,
Vasalli J,
Reich E
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.55
Subject(s) - concanavalin a , lymphokine , interleukin 2 , lymphocyte , mitogen activated protein kinase , immunology , microbiology and biotechnology , growth factor , t lymphocyte , mixed lymphocyte reaction , biology , chemistry , immune system , biochemistry , in vitro , t cell , receptor
Summary The continued proliferation of activated T cells requires the presence of a lymphocyte growth factor in the culture medium. This study describes a rapid, highly reproducible assay to quantitatively measure levels of this lymphokine. The use of Concanavalin‐A blast cells gives this assay a high degree of flexibility and convenience. It is shown that the lymphokine measured is Interleukin 2. The presence of an inhibitor in the supernatant of mitogen‐activated lymphocytes and the species specificity of the factor are demonstrated.