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ASCORBIC ACID IN INTESTINAL TISSUES
Author(s) -
Oelrichs BA,
Kratzing CC
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.53
Subject(s) - ascorbic acid , incubation , chemistry , saline , small intestine , biochemistry , phosphate buffered saline , biology , chromatography , endocrinology , food science
Summary About a fifth of the ascorbic acid is readily lost from intestinal tissue during handling procedures such as washing with saline and blotting. Further losses occur during incubation in Krebs‐phosphate saline; after 10 min strips of intestine retained 80% of their original ascorbic acid content and chopped tissue only 50%. This suggests that some of the intestinal ascorbic acid is very loosely held in the tissue. The small intestine is capable of accumulating double the normal amount of ascorbic acid when animals are dosed intramuscularly and retains some ascorbic acid (0·7 to 5 μg/mg DNA) even when the animals' body stores are depleted.