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PYRIDINE NUCLEOTIDE INVOLVEMENT IN RAT HEPATIC DRUG METABOLISM: PYRIDINE NUCLEOTIDE KINETICS OF ANILINE PARAHYDROXYLATION
Author(s) -
Penglis Stan,
Gourlay Geoffrey K,
Stock Beresford H
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.52
Subject(s) - aniline , reductase , chemistry , microsome , cytochrome , phenobarbital , nucleotide , kinetics , metabolism , biochemistry , pyridine , drug metabolism , enzyme , pharmacology , medicinal chemistry , biology , organic chemistry , physics , quantum mechanics , gene
Summary Apparent kinetic constants (NADPH) have been calculated for NADPH‐cytochrome P 450 reductase and parahydroxylase in the presence of aniline with hepatic microsomes from both phenobarbital pre‐reated and untreated rats. The addition of NADH gave similar stimulation of both aniline parahydroxylation and NADPH‐cytochrome P 450 reductase activity in the presence of aniline. It is proposed that the increase in aniline metabolism with NADH is due to an increase in the NADPH‐cytochrome P 450 reductase activity which is rate limiting in microsomes from both phenobarbital and untreated rats.