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PROPERTIES OF A MURINE LYMPHOKINE THAT AUGMENTS E‐ROSETTE FORMATION
Author(s) -
Morrison JJF,
Halliday WJ
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.49
Subject(s) - lymphokine , sensitization , in vitro , peripheral blood mononuclear cell , antigen , hapten , rosette (schizont appearance) , spleen , stimulation , microbiology and biotechnology , immunology , chemistry , rosette formation , biology , antibody , biochemistry , endocrinology
Summary Antigenic stimulation of spleen mononuclear cells from contact‐sensitized mice produced a soluble factor that enhanced the formation of E‐rosettes by human lymphocytes in vitro . Production of this murine E‐rosette augmenting factor (E‐RAF) occurred over a narrow range of antigen concentrations, was hapten‐specific, and depended on T‐lymphocytes. The factor was not dialysable, in contrast to other substances extracted from normal leucocytes. Murine E‐RAF is thus a lymphokine whose action is not species restricted. It was readily detected 30 min after contact of cells with antigen. E‐RAF formation by spleen cells and delayed‐type hypersensitivity reactions were elicited over a similar period after contact sensitization of mice.