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EFFECT OF 5‐AZACYTIDlNE ON DNA METHYLATION IN EHRLICH'S ASCITES TUMOR CELLS
Author(s) -
Tanaka M,
Hibasami H,
Nagai J,
Ikeda T
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.39
Subject(s) - dna , dna methylation , in vitro , methylation , microbiology and biotechnology , dna synthesis , neoplasm , in vivo , ascites , cancer research , chemistry , tumor cells , biology , biochemistry , medicine , genetics , gene , gene expression
Summary 5‐Azacytidine inhibited in vivo DNA methylation in Ehrlich's ascites tumor cells depending upon the dose at which 5‐azacytidine did not inhibit DNA synthesis significantly. This drug did not inhibit DNA methylation in vitro . The DNA methylase activity in ascitic cells decreased with the increasing dose of 5‐azacytidine. Hypomethylated DNA was obtained from the 5‐azacytidine treated ascitic cells.