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A RAPID AND EFFICIENT PROCEDURE FOR THE REMOVAL OF NONVIABLE CELLS FROM SUSPENSION CULTURE OF LYMPHOID CELL LINES
Author(s) -
Hurrell John GR,
Black Paul H,
Haber Edgar,
Zurawski Vincent R
Publication year - 1980
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1980.36
Subject(s) - cell culture , ficoll , centrifugation , lymphatic system , cell , biology , suspension culture , differential centrifugation , microbiology and biotechnology , chemistry , immunology , genetics , in vitro , biochemistry , peripheral blood mononuclear cell
Summary The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 × g on a Ficoll‐Hypaque (FH) cushion is described. Viable cells collect at the medium‐FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.