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GEL ELECTROPHORESIS OF ROTAVIRUS RNA DERIVED FROM SIX DIFFERENT ANIMAL SPECIES
Author(s) -
Smith M,
Tzipori S
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.61
Subject(s) - rotavirus , foal , rna , biology , polyacrylamide gel electrophoresis , virology , gel electrophoresis , virus , microbiology and biotechnology , reoviridae , genetics , gene , biochemistry , enzyme
Summary Rotavirus RNA prepared from calf, pig, mouse, deer, foal and dog‐adapted human isolates was compared using polyacrylamide gel electrophoresis. Reproducible differences in the RNA migration patterns, were found between all isolates. There were 11 clearly resolved segments in the pig, mouse and foal samples. The calf rotavirus RNA and deer rotavirus RNA separated into 9 bands and 10 bands, respectively. The dogs‐adapted human virus migrated in 12 bands, and this probably mulls from the complex passage history of the original human rotavirus isolate.