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NORADRENERGIC TRANSMISSION IN ISOLATED GUINEA‐PIG INTESTINE FOLLOWING IN VIVO ADMINISTRATION OF ANTIBODIES TO DOPAMINE β‐HYDROXYLASE
Author(s) -
Furness JB,
Costa M,
Rush RA,
Geffen LB
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.21
Subject(s) - guinea pig , dopamine , in vivo , antibody , transmission (telecommunications) , pharmacology , endocrinology , chemistry , biology , immunology , computer science , telecommunications , microbiology and biotechnology
Summary The effectiveness of transmission from noradrenergic nerves supplying the guinea‐pig ileum was evaluated in normal preparations and in preparations taken from animals injected 18 h to 4 days previously with antiserum to dopamine β‐hydroxylase. Degeneration of the nerves following the antiserum was monitored histochemically in the same preparations. A decline in the effectiveness of transmission, which paralleled the degeneration of the nerves, was observed. The earliest effects were detected at 18 h and the greatest effect was found at 2–4 days following the administration of antiserum. Binding of the antibodies has been detected as early as 6 h after injection. It is therefore concluded that binding of the antibodies to the nerves, per se , does not significantly compromise transmission, nod that histochemical evidence of degeneration can be used to indicate the onset of functional deterioration of noradrenergic nerves following their exposure to antibodies to dopamine β‐hydroxylase.