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GENERATION OF HOMOGENEOUS POPULATIONS OF ALLOREACTIVE T CELLS IN VITRO
Author(s) -
Woolnough James A,
Lafferty Kevin J
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.13
Subject(s) - homogeneous , in vitro , microbiology and biotechnology , biology , physics , genetics , statistical physics
Summary The supernatant from Con A‐activated spleen cells (CS) can be used to generate homogeneous populations of alloreactive T cells in vitro . Subculture of activated cells in CS containing medium is required for the continued proliferation and expression of effector activity. Prolonged Subculture in CS containing medium does not result in indefinite growth and proliferation of alloreactive T cells. The activity in CS required to maintain cytotoxic cell growth is not species specific, and is therefore separable from the costimulator activity in CS required for the initiation of the T cell response to alloantigen; this latter activity is species specific.