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EFFECT OF TREATMENT WITH AZATHIOPRINE ON THE RESPONSES OF RAT LYMPHOCYTES TO PHYTOHAEMAGGLUTININ
Author(s) -
Mansour A,
Nelson DS
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.12
Subject(s) - phytohaemagglutinin , spleen , azathioprine , in vitro , in vivo , immunology , medicine , endocrinology , peripheral blood , pharmacology , biology , biochemistry , microbiology and biotechnology , disease
Summary The proliferative responses of rat peripheral blood lymphocytes (PBL) and spleen tells to phytohemagglutinin (PHA) were studied after single or multiple (daily for 4 days) injections of azathioprine (AZ). Lymphopenia developed within 4 h of a single dose (78 mg/kg) of AZ and persisted for at least 72 h. There was no lymphopenia 24 h after the last of 4 daily injection. In vitro . PBL were more sensitive than spleen cells to the inhibitory effect of AZ. Likewise, the responses of PBL were relatively more depressed than those of spleen cells after single or multiple injections of AZ. The degree of depression was less than was expected from the effect of AZ in vitro . Multiple small doses were more depressive than multiple large closes. Serum from treated rats used at 20% concentration, was more depressive than normal. Thus, rat Lymphocytes are quite sensitive to AZ in vitro , but appear to be relatively resistant in vivo , this resistance resembling the resistance of the primary antibody response to AZ treatment.