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ENHANCEMENT OF NITROBLUE TETRAZOLIUM DYE REDUCTION BY LEUCOCYTES EXPOSED TO A FACTOR RELEASED FROM SENSITISED LYMPHOCYTES
Author(s) -
Oleske JM,
Ashman RB,
Kohl S,
Nahmias AJ
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.11
Subject(s) - nitroblue tetrazolium , in vitro , immunology , formazan , antigen , chemistry , microbiology and biotechnology , biology , biochemistry
Summary Leucocytes from purified protein derivative (PPD) skin test positive individuals, after exposure to PPD in vitro . show a significant increase in nitroblue tetrazolium (NBT) dye reduction when compared to leucocytes from PPD negative donors. The active principle was shown to be soluble, and was released from lymphocytes after a rapid, immunologically specific interaction with the sensitising antigen.