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SEARCH FOR HUMAN TUMOUR VIRUSES BY TRANSFECTION: UPTAKE OF MELANOMA AND EPSTEIN‐BARR VIRUS DNA BY HUMAN CELLS
Author(s) -
Brown SC,
Parsons PG,
Pope JH
Publication year - 1979
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1979.1
Subject(s) - transfection , virology , rous sarcoma virus , virus , biology , retrovirus , dna , cell culture , lymphoblast , microbiology and biotechnology , genetics
Summary In a model system, consistent transfection of chick embryo fibroblasts (CEF) by DNA from the XC cell line occurred, with recovery of infections Rous sarcoma virus. The techniques were then applied in attempts to recover possible human humour viruses. Even with various modifications of the XC technique, DNA from three human malignant melanoma cell lines failed to infect adult or foetal human fibroblasts, although melanoma DNA was taken up into nuclei of target cells. XC DNA did not transfect human foetal fibroblasts and melanoma DNA was ineffective in CEF. DNA from the Raji (Epstein‐Barr virus non‐producer) and QIMR‐WIL (producer) lymphoblastoid cell lines applicable techniques therefore failed to recover EB virus the putative melanoma retrovirus, or other potential tumour virus.