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A METHOD FOR THE ASSAY OF ABLASTIN IN THE SERUM OF RATS INFECTED WITH TRYPANOSOMA LEWISI
Author(s) -
Ferrante A,
Drew PA,
Jenkin CR
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.81
Subject(s) - biology , titer , parasite hosting , trypanosoma , virology , in vitro , microbiology and biotechnology , biochemistry , virus , world wide web , computer science
Summary This paper describes a simple quantitative assay for ablastin, a factor present in the serum from rats infected with Trypanosoma lewisi . which prevents the division of the parasite. The assay measures in vitro the inhibition of the incorporation of 3 H‐TdR into the DNA of T. lewisi in the presence of serum from infected animals. Utilising this method, one can measure the titre of ablastin in a particular serum sample and the time and duration of its appearance in the circulation of infected rats.