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THE USE OF MARSUPIAL X EUTHERIAN SOMATIC CELL HYBRIDS TO STUDY MARSUPIAL CELL SURFACE ANTIGENS
Author(s) -
Sykes Pamela J,
Hope Rory M
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.77
Subject(s) - biology , antigen , somatic cell , antiserum , marsupial , chromosome , microbiology and biotechnology , immunofluorescence , genetics , antibody , gene , zoology
Summary Buck and Bodmer (1976) have developed a technique for identifying an antigen on the surface of human x mouse somatic cell hybrids, specified by a gene on a particular human chromosome. We have successfully adapted this technique to a study of marsupial cell surface antigens. Somatic cell hybrids between Macropus rufus (Marsupialia) lymphocytes and the mouse cell lines PG19 and 1R were injected intraperitoneally into mice of the same inbred strain from which the above cell lines were derived (C57Bl6J and C3H, respectively). The only identified M. rufus chromosome present in the hybrid cells was the X chromosome. The antisera, after adsorption with PG19 or 1R, were tested using indirect immunofluorescence, against the hybrid cells, and also against sub‐clones (derived from the hybrids) which had apparently lost the M. rufus X chromosome, or at least its long arm. The results of these tests showed that the adsorbed antisera contained reactivity against an M. rufus cell surface antigen (or antigens). The reactions of one of the antisera were most simply interpreted by supposing that it was detecting an M. rufus X‐linked antigen(s).