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IN VITRO DEGRADATION OF MOUSE, RABBIT AND DOG ANTIBODIES TO VIBRIO CHOLERAE BY SUCCUS ENTERICUS
Author(s) -
Horsfall DJ,
Heddle RJ,
Cormly AA,
Rowley D
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.75
Subject(s) - vibrio cholerae , antibody , microbiology and biotechnology , in vitro , biology , proteolysis , cholera , digestion (alchemy) , chemistry , bacteria , immunology , enzyme , biochemistry , genetics , chromatography
Summary Purified antibodies to Vibrio cholerae from mouse, rabbit and dog were digested in vitro by homologous intestinal secretions. When assessed with regard to their complement‐dependent vibriocidul activity. IgG antibodies were generally more susceptible to degradation than IgM antibodies. High levels of tryptic inhibitors were required to inhibit this digestion. Rabbit IgG was unusual in being quite resistant to digestion. Gel filtration studies demonstrated that secretory IgA, isolated from mouse intestinal secretions, was resistant to proteolysis. Similar studies on dog IgG and mouse IgM demonstrated production of F(ab') 2 ‐like fragments. Digests of these antibodies, while devoid of Fc‐mediated vibriocida) activity, retained significant protective activity for baby mice.