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TRANSFER OF MOUSE IgG 2 PRODUCTION BY IgM‐BEARING SPLEEN CELLS SEPARATED BY A FLUORESCENCE‐ACTIVATED CELL SORTER
Author(s) -
Bankhurst Arthur D,
Anderson Robert E,
Scott Cram L,
Horan Paul H,
Warner Noel L
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.63
Subject(s) - allotype , spleen , antibody , microbiology and biotechnology , surface immunoglobulin , antiserum , b cell , isotype , congenic , biology , cell , immunology , immunoglobulin m , chemistry , immunoglobulin g , biochemistry , monoclonal antibody , gene
Summary The purpose of the present study was to determine whether at least some splenic B lymphocytes can switch from the synthesis of one isotype of immunoglobulin to another during B cell differentiation. The experimental system involved the transfer of characterized cell suspensions between allotype congenie strains of mice followed by analysis in the recipient for donor type immunoglobulin production. Donor splenic lymphocytes were incubated with specific fluorescent labelled anti‐μ antiserum and passed through the Los Alamos fluorescence‐activated cell sorter; μ‐depleted cell suspensions were transferred into sublethally irradiated congenic recipients and the amount of donor type immunoglobulin of IgG 2 type was measured at weekly intervals. The results demonstrated that at least some cells hearing membrane bound IgM can differentiate in vivo into IgG 2 ‐secreting cells, although not all IgG 2 ‐secreting cells have been recently derived from IgM positive precursors.