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CONTROL OF CONCANAVALIN A RECEPTOR MOBILITY BY CYTOPLASMIC ACTIN IN HUMAN TUMOUR CELLS
Author(s) -
Koh SH,
Toh BH,
Gallichio HA,
Elrick WL
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.33
Subject(s) - staining , cytoplasm , cytochalasin , cell , biology , cytochalasin b , microfilament , cytochalasin d , actin , microbiology and biotechnology , pathology , cytoskeleton , biochemistry , medicine , genetics
Summary Tissue culture monolayers of seven human intracranial tumours comprising 2 astrocytomas, 3 meningiomas, 1 secondary squamous cell carcinoma and 1 secondary adenocarcinoma were examined by a double immunofluorescent staining technique to demonstrate Concanavalin A (Con A) surface receptors and cytoplasmic actta in the same cell. Tumour cells, treated with fluoresceinisothiocyanate‐labelled Con A (FITC‐Con A) showed staining in cell margins or in a random distribution over the cell surface. Incubating the cells with FITC‐Con A at 37 ° for increasing periods of time resulted first in staining of clusters and later of perinuclear globules. Cells, preteated with 4% paraformaldehyde at 4 ° for 10 min or with cytochalastn B at 37 ° for 30 min showed Staining restricted to cell margins. In the cytochalasin B‐treated cells, tile peripheral staining was in the form of coarse clusters. Double fluorochrome studies showed that the anti‐action antibody (AAA) staining occurred in sites closely related to those stained by FITC‐Con A both in untreated as well as in cytochalsin B‐treated cells. The findings suggest that Con A receptors, as an example of a stable cell membrane determinant in human tumour cells, are associated with actin and that their mobility on the cell surface is dependent on an intact cytoplasmic actin system.