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EFFECT OF HYDROCORTISONE ON THE RESPONSE OF RAT LYMPHOCYTES TO PHYTOHAEMAGGLUTININ
Author(s) -
Mansour A,
Nelson DS
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.32
Subject(s) - phytohaemagglutinin , hydrocortisone , biology , medicine , psychology , endocrinology , immunology , lymphocyte
Summary The proliferative response of nit peripheral blood lymphocytes and spleen cells to phytohaemagglutinin (PHA) was studied following treatment with single or multiple doses of hydrocortisone. A transient lymphopaenia with a rapid recovery at 24 h occurred with both dose schedules. Hydrocortisone in vitro caused a dose‐dependent depression of blood lymphocyte response to PHA but enhanced the response of spleen cells when the steroid was added after the mitogen. An inverse relationship between blood and splenic lymphocyte responses to PHA occurred after a single dose of hydrocortisone. Blood lymphocytes from multiple dose hydrocortisone‐treated rats had an enhanced response to PHA. Serum collected within 5 min of injection from single dose‐treated rats depressed normal rat blood lymphocyte responses. After multiple doses of hydrocortisone serum enhanced blood lymphocyte responses to PHA. The primary antibody response to sheep erythrocytes was only affected by mar toxic doses of hydrocortisone.