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STUDIES ON THE BRUSH BORDER MEMBRANE OF MOUSE DUODENUM: LIPIDS
Author(s) -
Billington T,
Nayudu PRV
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.3
Subject(s) - phospholipid , sphingomyelin , chloroform , chemistry , chromatography , cerebroside , ethanolamine , glycolipid , cholesterol , biochemistry , brush border , membrane , vesicle
Summary Lipids were extracted from purified mouse duodenal brush border membranes. Lipid:protein ratios in different membrane preparations varied from 0·58 to 0·68. Both the chloroform and non‐chloroform phases were quantitatively analysed for lipids. Chloroform extracts were composed of cholesterol, triglycerides and phospholipids. The major neutral lipid was cholesterol. Rechromatrography of the phospholipid spot showed sphingomyelin (7·9%), phosphatides of ethanolamine (61·7%), inositol (14·2%) and serine (16·2%). The average molar ratio of cholesterol to phospholipid was 1·39. Lipids in the non‐chloroform phase were all glycosylated, being cerebrosides (69·3%), cerebroside sulphates (28·8%) and galgliosides (1·9%). Overall membrane lipid composition was neutral lipid 24%, phospholipid 33%, and glycolipid 43%.