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ISOLATION OF BACTERIOPHAGE‐LIKE PARTICLES FROM UNINDUCED CLOSTRIDIUM TETANI CULTURES
Author(s) -
Brown Kurt J,
Brown Patricia A
Publication year - 1978
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1978.15
Subject(s) - clostridium tetani , bacteriophage , isolation (microbiology) , microbiology and biotechnology , biology , clostridium , virology , bacteria , escherichia coli , genetics , tetanus , gene , vaccination
Summary The isolation of hexagonal‐headed, tailless, bacteriophage‐like particles from uninduced cultures of Clostridium tetani is described. Clear, round. 1‐3 mm diameter plaques were noted on Clostrisel agar plates, which were overlaid with soft agar inoculated with 7‐14 day broth cultures. Particles were detected by transmission electron microscopy from broth cultures seeded with scrapings from the plaques. Both election dense and electron lucent heads were noted. An electron dense head was observed attached to the surface of a dividing bacterium.