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REQUIREMENTS FOR STIMULATION OF T CELL RESPONSES AGAINST VIRUS‐INFECTED CELLS: NATURE OF ECTROMELIA VIRUS‐INFECTED CELLS CAPABLE OF STIMULATING CYTOTOXIC T CELLS IN A SECONDARY RESPONSE IN VITRO
Author(s) -
Pang T,
Blanden RV
Publication year - 1977
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1977.53
Subject(s) - ectromelia virus , cytotoxic t cell , stimulation , in vitro , virus , virology , biology , ectromelia , vaccinia , neuroscience , biochemistry , gene , recombinant dna
Summary The nature of infected stimulator cells in the in vitro secondary cytotoxic T cell response to ectromelia infection was investigated. It was found that macrophages were better stimulator cells than spleen cells. B cells (Ig‐positive cells) were superior to T cells (Ig‐negative cells) both on a relative proportion and on a cell‐to‐cell basis. Concanavalin A and lipopolysaccharide‐stimulated lymphocytes were also effective stimulator cells but appeared to be slightly inferior to spleen cells. Spleen cells depleted of la‐positive cells were markedly inferior to normal spleen cells as stimulators. It was also found that primary and secondary cytotoxic T cells were largely Ia‐negative. These findings are discussed in relation to the likely events during T cell responses to infection in vivo.