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THE RESPONSE OF FOETAL SHEEP TO THE SOMATIC AND FLAGELLAR ANTIGENS OF SALMONELLA TYPHIMURIUM
Author(s) -
Fahey Kevin J
Publication year - 1977
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1977.52
Subject(s) - salmonella , somatic cell , antigen , microbiology and biotechnology , biology , somatic antigen , virology , immunology , genetics , bacteria , gene
Summary Following the injection of polymeric flagellin (POL), foetal sheep older than 70 days gestation produced haemagglutinating. antibody and synthesized IgM. The maximum titre of antibody in the blood increased with the; age at which the foetus was injected. All foetuses synthesized 2‐meracapto‐ethanol‐sensitive antibodies, while older foetuses (approximately 120 days gestation) also produced 2‐mercaptoethanol‐resistant antibodies and synthesized IgG 1 . During the primary immune response, there was a poor correlation between the antibody titre and the amount of immunoglobulin synthesized. The majority of IgM synthesized and almost all IgG 1 , had no demonstrable specificity for POL. During the secondary response to POL the majority of IgG 1 synthesized was specific and in one case appeared to be monoclonal. There was no detcetable' primary antibody response in foetal sheep to the somatic antigens of Salmonella typhimurium , although all foetuses synthesized IgM. Only one of six: foetuses receiving a second injection of antigen produced antibody. There was an increase in the numbers of blood lymphocytes following the injection of both POL and S. typhimurium , but only POL induced a rapid increase in the numbers of neutrophils in the blood and produced histological changes in the draining lymph nodes and spleen.