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INCORPORATION OF [2‐ 3 H] ETHANOLAMINE INTO RAT MUSCLE PHOSPHOGLYCERIDES
Author(s) -
Shamgar Felix A,
Collins Frederick D
Publication year - 1977
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1977.33
Subject(s) - phosphatidylethanolamine , phosphatidylcholine , biochemistry , chemistry , ethanolamine , methylation , biosynthesis , chromatography , phospholipid , enzyme , membrane , dna
Summary The contribution of phosphatidylethanolamine methylation to phosphatidylcholine biosynthesis in rat muscle was investigated by studying the incorporation of [2‐ 3 H] ethanolamine. The specific radioactivities of individual molecular species of muscle phosphoglycerides were measured by a combination of argentation thin‐layer chromatography and countercurrent distribution. The specific radioactivity of phosphatidylethanolamine was approximately one thousand times that of phosphatidylcholine. Amongst individual phosphatidylethanolamines, hexaenoic species possessed the highest specific radioactivities and tetracnoic the lowest. Because of the very low incorporation into phosphatidylcholine, the specific radioactivities of combined rather than of individual fractions were measured. The results indicate that the contribution of phosphatidylethanolamine methylation to the overall biosynthesis of phosphatidylcholine in muscle is of minor importance.