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ENDO‐ AND EXOGLYCOSIDASES IN AN EXPERIMENTAL RAT OSTEOSARCOMA
Author(s) -
Harris PA,
Stephens RW,
Ghosh P,
Taylor TKF
Publication year - 1977
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1977.32
Subject(s) - osteosarcoma , chemistry , biology , cancer research
Summary Glycosidases capable of degrading intercellular matrix components were investigated in a 32 P induced rat osteosarcoma. Homogenates of ossifying tumour were shown to readily degrade hyaluronic acid, chondroitin sulphates 4 and 6 but not dermatan sulphate. High levels of the exoglycosidases, β‐glucuronidase and β‐N‐acetylgluco‐saminidase were found in tumour homogenates, and it was demonstrated that these enzymes contribute to the degradation of high molecular weight hyaluronic acid. The levels of these enzymes were compared with activities found in homogenates of neonatal bone and muscle surrounding tumours. Exoglycosidases, but not hyaluronidase, were found to be produced by cultures of osteosarcoma in vitro.