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MICELLES OF CEREBROSIDE SULPHATE
Author(s) -
Jeffrey Hilary J,
Roy Alexander B
Publication year - 1977
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1977.28
Subject(s) - micelle , chemistry , ionic strength , ultracentrifuge , chromatography , critical micelle concentration , sodium formate , sephadex , cerebroside , substrate (aquarium) , sodium , inorganic chemistry , biochemistry , organic chemistry , enzyme , aqueous solution , oceanography , geology
Summary The critical micelle concentration of cerebroside sulphate in water is 0·01 mM: it increases with increasing concentrations of buffer to 0·07 mM in 0·1 M sodium acetate and formate buffers. pH 5·6 and 4·5 respectively. The partial specific volume of the micelles is about 0·94. The behaviour of the micelles in the ultracentrifuge and on Sephadex G‐200 shows them to he grossly heterogeneous with respect to size. In 0·1 M buffer s 20, w is about 26 S; in water or 0·01 M buffer smaller micelles with an s 20, w . of about 6 S are also present. In 0·01 M formate, pH 4·5, the smallest species detectable by equilibrium ultracentrifugation had a micellar weight of about 180,000 corresponding to an aggregation number of about 180. Much larger aggregates were also present. It is suggested that the smallest micelles are the substrate for sulphatase A when this is acting as a cerebroside sulphatase in buffers of low ionic strength.