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ASSAY OF HUMAN LYMPHOKINES IN VITRO . EVIDENCE FOR A MIGRATION STIMULATION FACTOR (MStF) WHICH INTERFERES WITH THE MACROPHAGE MIGRATION INHIBITION ASSAY
Author(s) -
Aaskov JG,
Anthony Honor M
Publication year - 1976
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1976.54
Subject(s) - lymphokine , macrophage migration inhibitory factor , stimulation , in vitro , chemotaxis , macrophage , macrophage activating factor , cell migration , chemotaxis assay , antigen , biology , chemistry , immunology , microbiology and biotechnology , endocrinology , cytokine , biochemistry , receptor
Summary When cultured in vitro , human lymphocytes produced a range of materials which were able to reduce the migration inhibitory effect of human macrophage migration inhibition factor (MIF) and in some cases stimulated macrophage migration to greater than control levels. In addition, lymphocytes stimulated with antigen or mitogen produced a lymphokine‐like migration stimulation “factor”. Detectable production of this factor occurred 24–48 h after antigen stimulation. The migration stimulation factor (MStF) (MVV 50–250,000; pI 6·5) was not chemotactic and could be separated from migration inhibition and chemotactic factors by chromatography and iso‐electric focussing.