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PREPARATION AND TESTING OF GOAT ANTI‐THYMOCYTE GLOBULIN
Author(s) -
Mears DC,
Sheil AGR,
Kelly GE,
Beerenfels PC
Publication year - 1976
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1976.43
Subject(s) - thymocyte , globulin , antibody , fractionation , absorption (acoustics) , chemistry , toxicity , immunology , biology , chromatography , microbiology and biotechnology , medicine , immune system , physics , acoustics , cd8
Summary This study details methods of production, purification and testing of large volumes of uniformly potent, non‐toxic, anti‐human thymocyte globulin (ATG), Plasma from 70 goats immunised with human thymic lymphocytes was fractionated by ammonium sulphate precipitation to its IgG component. Contaminating antibodies were removed by absorption methods. After fractionation and absorption, ATG retained its ability to inhibit the formation of rosettes by human lymphocytes with sheep red blood cells. Antibodies to human glomerular basement membrane were not detected in any tested preparation. Tests performed on monkeys, mice and rabbits revealed low toxicity.