Premium
CYTOTOXIC T CELLS IN THE PERITONEAL CAVITY OF MICE INFECTED WITH ECTROMELIA VIRUS
Author(s) -
Pang T,
Gardner ID,
Blanden RV
Publication year - 1976
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1976.36
Subject(s) - ectromelia virus , ectromelia , cytotoxic t cell , virology , peritoneal cavity , virus , biology , anatomy , in vitro , genetics , gene , vaccinia , recombinant dna
Summary Peritoneal exudate cells from mice infected with ectromelia virus were cytotoxic for virus‐infected target cells as measured in a 51 Cr release assay. Cytotoxic activity seemed to be T cell‐dependent as it was largely abolished by treatment with anti‐θ serum and complement but was not impaired by macrophage depletion. The kinetics of development of cytotoxicity in the peritoneal cavity lagged behind spleen cytotoxicity by 1–2 days. Peak activity in peritoneal cells was present about 6 days after intravenous infection with virus. These studies suggest that macrophages present in the free peritoneal cell populations of ectromelia‐infected mice are not cytotoxic for virus‐infected target cells. The effect of macrophages in virus clearance is therefore likely to be due to phagocytic rather than cytotoxic effects.