CYTOPHILIC ATTACHMENT OF OVINE IgG 2  TO AUTOLOGOUS POLYMORPHONUCLEAR LEUCOCYTES | Zendy

Watson DL | Zendy

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CYTOPHILIC ATTACHMENT OF OVINE IgG 2 TO AUTOLOGOUS POLYMORPHONUCLEAR LEUCOCYTES

Author(s) -

Watson DL

Publication year - 1975

Publication title -

australian journal of experimental biology and medical science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.999

H-Index - 104

eISSN - 1440-1711

pISSN - 0004-945X

DOI - 10.1038/icb.1975.57

Subject(s) - incubation , staining , antibody , microbiology and biotechnology , chemistry , incubation period , immunology , biology , biochemistry , pathology , medicine

Summary Preparations of ovine polymorphs were stained with fluorosceinconjugated, monospecific anti‐immunoglobulin sera. Approximately 26% of polymorphs specifically stained with anti‐ γ 2 , 16% with anti‐light chain, 24% with anti‐Fab and 24% with anti‐ovine serum. There was no significant staining of polymorphs with anti‐ γ 1 , anti‐ μ or anti‐ α . Cytophilic IgG 2 could be removed from the cells by incubation at 37° and subsequently replaced by incubation in autologous serum at 4°.

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