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CYTOPHILIC ATTACHMENT OF OVINE IgG 2 TO AUTOLOGOUS POLYMORPHONUCLEAR LEUCOCYTES
Author(s) -
Watson DL
Publication year - 1975
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1975.57
Subject(s) - incubation , staining , antibody , microbiology and biotechnology , chemistry , incubation period , immunology , biology , biochemistry , pathology , medicine
Summary Preparations of ovine polymorphs were stained with fluorosceinconjugated, monospecific anti‐immunoglobulin sera. Approximately 26% of polymorphs specifically stained with anti‐ γ 2 , 16% with anti‐light chain, 24% with anti‐Fab and 24% with anti‐ovine serum. There was no significant staining of polymorphs with anti‐ γ 1 , anti‐ μ or anti‐ α . Cytophilic IgG 2 could be removed from the cells by incubation at 37° and subsequently replaced by incubation in autologous serum at 4°.