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VIBRIO CHOLERAE INFECTION AND IMMUNITY IN MICE
Author(s) -
Lee Margaret M,
Lee A,
Cooper GN
Publication year - 1975
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1975.41
Subject(s) - vibrio cholerae , immunity , microbiology and biotechnology , cholera , biology , virology , immunology , immune system , bacteria , genetics
Summary Lyophilized cultures of V. cholerae 569B slowly lose their virulence for neonatal and adult mite during long term storage. Following a single passage in orally infected 6‐day old mice, a highly virulent strain (designated 569B/MP) was isolated. This organism causes rapidly fatal intestinal infections in 6‐day old mice; large numbers can he isolated in pure culture from the intestinal fluid. Freezing and storage at −60° of dead animals provides a simple means of maintaining the high virulence of the culture over a period of at least 9 months. This strain produces choleraic symptoms and death in approx. 50% of adult mice following oral infection. Large numbers of viable organisms may also be isolated from the small intestine over a period of at least 40 h. These criteria have been used as a basis for assessing protection against cholera infection induced by immunization with living or heat‐killed V. cholerae given orally or intraperitoneally.