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RAPID DETECTION AND ISOLATION OF MYCOPLASMAS FROM CELL CULTURES
Author(s) -
Tomkins GA,
MacGregor A,
Pye D,
Atkinson Mary
Publication year - 1975
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1975.28
Subject(s) - mycoplasma , isolation (microbiology) , lincomycin , microbiology and biotechnology , uracil , uridine , antibiotics , cell culture , biology , chromatography , chemistry , biochemistry , dna , rna , genetics , gene
Summary An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.