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THE GENERATION OF LARGE PYRONINOPHILIC CELLS IN THE LYMPHOID TISSUES OF RATS INFUSED WITH CELL‐FREE LYMPH
Author(s) -
Yamashita A,
Fukumoto T,
Nawa Y,
Kotani M,
Fujii H,
Era I
Publication year - 1975
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1975.2
Subject(s) - lymph , lymphatic system , pathology , cell , medicine , chemistry , biochemistry
Summary The thoracic duct of Wistar strain rats was cannulated during 5 days fur studying the effect of selective lymphocyte depletion on the lymphoid tissue. A technique for the continuous infusion of cell‐free lymph, whole lymph or Eagle's medium to the rat with the thoracic duct fistula is described in detail. The prolonged drainage of lymph from rats was followed by lymphopenia, severe atrophy of lymphoid tissues and the depletion of small lymphocytes in the thymus‐dependent areas of spleen and lymph nodes. The infusion of cell‐free lymph into the drained rat resulted in the recovery of the weight of lymphoid tissues and in the massive proliferation and accumulation of large, cells with prominent nucleoli and intensely pyroninophilic cytoplasm in the lymphocyte depleted areas of the peripheral lymphoid tissues and thymic cortex. There was histological evidence that the large pyroninophilic cells developed well in the spleen and tended to localize preferentially around the periarteriolar region through the marginal zone bridging channels to the red pulp, The infusion of Eagle's medium was found ineffective in restoring the weight of the lymphoid tissues and in bringing about the proliferation of lymphoid cells. The rats infused with whole lymph showed almost similar findings biologically and histologically to those of sham‐operated rats.