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THE EFFECT OF IMMUNIZATION WITH A ROUGH STRAIN OF SALMONELLA ENTERITIDIS ON THE FATE OF SALMONELLA TYPHIMURIVM IN MICE
Author(s) -
Davies R,
Kotlarski I
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.77
Subject(s) - salmonella , salmonella enteritidis , microbiology and biotechnology , immunization , spleen , antigen , population , lipopolysaccharide , in vitro , biology , hemagglutination , immunity , antibody , virology , immune system , immunology , bacteria , medicine , biochemistry , genetics , environmental health
Summary Mice were immunized with living Salmonella enteritidis and then challenged at various tunes up to 20 weeks with Salmonella typhimurium C5. The resistance or the immunized mice at the time of challenge was determined by survival of these mice for a period of 28 days or by comparison of liver, spleen and blood counts of the challenge population obtained from normal and immunized mice. Results of survival experiments indicated that the immunization produced effective and long lasting immunity, although bacterial enumeration studies showed that the level of resistance induced was not sufficient to achieve complete, rapid elimination of the challenge organism from the tissues. Mice immunized with S. enteritidis 11RX showed both Arthus reactivity and delayed type hypersensitivity to S. typhimurium C5 antigens, and the serum from these mice was shown to contain antibody activity, as determined by haemagglutination of sheep erythrocytes coated with S. typhimurium C5 lipopolysaccharide and in vitro bactericidal action against S. typhimurium M206.