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SUBSURFACE LOCATION OF NON‐AGGLUTINATING ANTIGENS OF LEPTOSPIRA INTERROGANS (SEROTYPE BIFLEXA )
Author(s) -
Graves S,
Faine S
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.61
Subject(s) - antiserum , leptospira interrogans , leptospira , serotype , antigen , antibody , biology , microbiology and biotechnology , virology , immunology
Summary Neither monoprecipitin rabbit antiserum against precipitating antigen ‘d’ nor ‘e’ of Leptospira interrogans (serotype biflexa) would agglutinate whole leptospires, even at very low antiserum dilution. By means of antibody fluorescent labelling and anti‐globulin cross‐linking techniques, antigen ‘d’ and ‘e’ were shown to be subsurface in cellular location.