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TRYPSIN EXTRACTABLE PRECIPITATING ANTIGENS OF LEPTOSPIRA INTERROGANS (SEROTYPE BIFLEXA )
Author(s) -
Graves S,
Faine S
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.60
Subject(s) - leptospira interrogans , antigen , serotype , trypsin , immunodiffusion , microbiology and biotechnology , antibody , leptospira , biology , virology , chemistry , immunology , enzyme , biochemistry
Summary Extraction of Leptospira interrogans (serotype, bifexa) with 0·2% trypsin yielded three precipitating antigens (‘axial filament’; antigen ‘d’; antigen ‘e’) as detected by immunodiffusion against rabbit antileptospiral serum. In contrast to ‘axial filament’ antigen, antigens ‘d’ and ‘e’ could be detected only in stationary phase leptospires. Protein antigen ‘d’ was purified and gave rise to monoprecipitin sera in rabbits. Monoprecipitin sera against both antigen ‘d’ and ‘e’ were also produced by immunizing rabbits with specific immune, precipitates in agar. Rabbit anti‐‘d’ and anti‐’ e’ immunoglobulins were initially of the 19S type but subsequently became solely 7S.