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CELL PROLIFERATION KINETICS OF ENDOGENOUS AND EXOGENOUS ERYTHROID SPLEEN COLONIES IN LETHALLY IRRADIATED MICE
Author(s) -
Hasthorpe Suzanne,
Hodgson GS
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.56
Subject(s) - endogeny , spleen , biology , labelling , mitotic index , mitosis , bone marrow , phenylhydrazine , immunology , microbiology and biotechnology , biochemistry , chemistry , medicinal chemistry
Summary Two types of spleen colony formation have been compared with respect to the kinetics of erythroid cells. Endogenous colonies were obtained using phenylhydrazine treatment followed by lethal total body irradiation and exogenous colonies derived from normal bone marrow transplanted into irradiated animals. Individual colonies of both endogenous and exogenous types were scored for 3 HTdR labelling index (LI), mitotic index (MI) and vincristine mitotic accumulation. Then; was no significant difference in kinetic behaviour of erythmid colonies in the endogenous compared with the exogenous situation. Erythroid colonies from both endogenous and exogenous sources were classified into three separate groups, “early”, “intermediate” and “late” on the basis of their morphological appearance. When this classification was later taken into account there were no differences in kinetic behaviour between “early”, “intermediate” and “late” colonies of endogenous as compared to the respective classes of exogenous colonies. Labelling indices of “early”, “intermediate” and “late” colonies fell into three distinct groups having mean corrected labelling indices of approximately 75%, 63% and 47%, respectively. The mean nuclear diameter of cells in “early” colonies was significantly greater than of cells in “intermediate” and “late” colonies, cells of the latter two having similar mean nuclear diameters. The only significant difference observed between the endogenous and exogenous colonies was that the erythroid to granulocytic colony ratio was considerably higher for endogenous colonies. This may be attributed to the presence of an environment more favourable to erythroid development in spleens of mice which have haemolytic anaemia.