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ANTIGEN‐INITIATED B LYMPHOCYTE DIFFERENTIATION
Author(s) -
Schlegel RA
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.47
Subject(s) - antigen , priming (agriculture) , in vivo , flagellin , immunology , in vitro , immune system , microbiology and biotechnology , mixed lymphocyte reaction , biology , chemistry , t cell , biochemistry , receptor , botany , germination
Summary The response to the hapten NIP (4‐hydroxy‐3‐iodo‐5‐nitrophenylacetic acid) presented on the carrier POL (polymerised bacterial flagellin) in culture was found to be basically similar to the response in vivo. Primary immunisation gave unusual kinetics, peaking at 3 days, whereas a secondary response gave a normal peak at 4‐5 days. The primary response was exclusively IgM, whereas the secondary response was exclusively IgM, exclusively IgG or a combination of the two, depending on the priming protocol. Studies with athymic mice and anti‐ θ treated T cells demonstrated that a primary IgM response could be obtained in the absence of T cells, although the presence of T cells in vitro did appear to enhance the response. The IgG response was totally dependent on T cells. The addition of in vivo flagellin‐primed T cells produced effective collaboration with NIP‐primed B cells in the production of an IgG response. Addition of β ‐mercaptoethanol to the culture medium failed to enhance either the primary IgM or secondary IgG response to NIP‐POL. The cell dose response curve of unprimed cells was non‐linear under normal conditions, but could be made linear by the addition of irradiated cells to produce a constant number of cells per culture. The antigen dose response curve gave a sharp optimum, with no significant difference between primed and unprimed cells.