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ACTIVATION OF THORACIC DUCT LYMPHOCYTES FROM CONGENITALLY ATHYMIC “NUDE” MICE BY MITOGENIC STIMULI IN VITRO
Author(s) -
Kearney JF,
Reade PC
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.18
Subject(s) - phytohaemagglutinin , pokeweed mitogen , concanavalin a , thoracic duct , lymphocyte , in vitro , population , lipopolysaccharide , immunology , t lymphocyte , chemistry , endocrinology , biology , medicine , biochemistry , lymphatic system , environmental health
Summary Thoracic duct lymphocytes from congenitally athymic “nude” mice were used as a source of B‐lymphocytes in a study of the activation of B‐cells by various mitogenic stimuli. Bacterial lipopolysaccharide produced a response in the “nude” lymphocyte population which was equivalent to that obtained with thoracic duct lymphocytes from normal littermates. The mitogens concanavalin A, phytohaemagglutinin and the mixed lymphocyte reaction produced a poor response in “nude” mouse thoracic duct lymphocytes while pokeweed mitogen which has been shown to activate both T‐ and B‐cells in other systems showed very little stimulatory activity on this relatively pure population of B‐lymphocytes.