Premium
THE LYSOSOMAL ENZYMES ACID PHOSPHATASE AND β ‐GLUCURONIDASE IN MUSCLE FOLLOWING A PERIOD OF ISCHAEMIA
Author(s) -
Shan AD,
Adams EP,
Courtice FC
Publication year - 1974
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1974.13
Subject(s) - acid phosphatase , glucuronidase , beta glucuronidase , ischemia , enzyme , alkaline phosphatase , medicine , endocrinology , phosphatase , chemistry , anabolism , biochemistry , myocyte , biology , gene expression , gene
Summary Histochemical and biochemical investigations have been carried out on the activities of acid phosphatase and β ‐glucuronidase in plantar muscle following a period of 6 hours' ischaemia of the hindpaw of the rabbit. The activities of these enzymes were followed from 2 hours to 28 days after the period of ischaemia. The activities of both enzymes were unaltered in the injured muscle at 2 hours after the period of ischaemia. While the biochemical activity of acid phosphatase was unchanged at 1 day after ischaemia, β ‐glucuronidase activity was significantly increased at this time. Three days after ischaemia the biochemical activities of both enzymes were significantly increased. These increased activities were shown histochemical to be associated with infiltrating mononuclear cells in the case of acid phosphatase and with infiltrating mononuclear cells and degenerating muscle fibres in the case of β ‐glucuronidase. Both enzymes were still significantly increased in the injured muscle at 7 days after ischaemia. At this time regenerative processes were evident in the muscle and particularly high levels of β ‐glucuronidase activity were visualized within muscle fibres. It is suggested that β ‐glucuronidase may have an anabolic role in the early regenerative processes of muscle. Some acid phosphatase activity was also visualized within muscle fibres at 7 days. The biochemical activities of both enzymes approached normal values in the injured muscle at 14 days after ischaemia but residual activities were still evident histochemically at 28 days. The levels of acid phosphatase and β ‐glucuronidase were also determined in leg lymph and blood plasma. There were increased levels of both enzymes in the lymph draining the injured hindpaw 2 hours after ischaemia. These increased values in lymph had returned to normal levels at 1‐7 days after ischaemia, indicating that the initial increases resulted from an increased permeability of the small blood vessels in the affected area.