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THE EFFECT OF MATERNAL MALNUTRITION ON THE PROGENY IN THE RAT. STUDIES IN VITRO OF ADIPOSE TISSUE LIPOGENESIS AND LIPOPROTEIN LIPASE ACTIVITY FOLLOWING FRUCTOSE FEEDING
Author(s) -
McLeod Katrine I,
Nestel PJ,
Goldrick RB
Publication year - 1973
Publication title -
australian journal of experimental biology and medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0004-945X
DOI - 10.1038/icb.1973.77
Subject(s) - lipogenesis , medicine , endocrinology , lipoprotein lipase , triglyceride , glyceride , adipose tissue , nefa , biology , glycerol , fructose , hypertriglyceridemia , insulin , fatty acid , cholesterol , biochemistry
Summary When female rats are fed a protein‐deficient diet (6% protein by weight) for 2 weeks prior to and during gestation, the offspring exhibit deficits in body weight, epididymal fat pad weight and the size and number of adipocytes which cannot be corrected by an adequate post‐natal diet. However, the plasma cholesterol, triglyceride and glucose concentrations are similar in the progeny of normally‐fed (control) and protein‐deficient (test) dams. Studies of adipose tissue lipogenesis mid lipoprotein lipase activity were carried out in the absorptive or fasted states in 10‐ or 30‐week‐old control and test progeny that had either been fed a chow diet or chow plus a 20% fructose supplement in the drinking water for 2 weeks. Fructose feeding produced similar rises in the plasma triglyceride concentration in test and control progeny, the rise being more evident in older progeny. With chow feeding alone, the incorporation in vitro of glucose into both glyceride‐glycerol and glyceride‐fatty acids per adipocyte was similar in test and control groups at each age. With one exception (basal incorporation into glycerol in older lest progeny), fructose feeding increased both basal and insulin‐stimulated incorporation of glucose into glyceride‐glycerol and glyceride‐fatty acid but to a significantly smaller extent in the test progeny. With the exception of fructose‐fed test progeny, there was a rise in baseline glycerol production with age in all groups tested in the absorptive State but a fall with age when tested in the fasted state. There was also a marked reduction of fatty acid synthesis with age in all groups, and the values obtained in the fasted state were significantly lower than those in the absorptive state. Release of adipose tissue lipoprotein lipase was similar in test and control progeny when expressed in terms of unit numbers of adipocytes and was unaffected by fructose feeding.